automated cell culture maintenance instrument that continuously images by microscopy live interphase cells, live mitotic cells, live differentiated cells, and dead cells in growing cell cultures and quantifies changes in their respective numbers during culture; use of cell count data to continuously determine the proliferation rate of cell cultures, the mitotic fraction of cell cultures, and the differential tissue stem cell count of primary tissue cell cultures; determination of differential tissue stem cell count of uncultured primary tissue cell samples

'autom':1 'cell':2,13,16,19,22,25,38,48,54,61,66,73,79 'chang':29 'continu':7,42 'count':39,62,74 'cultur':3,26,35,49,55,67 'data':40 'dead':21 'determin':43,68 'differenti':18,58,70 'fraction':52 'grow':24 'imag':8 'instrument':5 'interphas':12 'live':11,14,17 'mainten':4 'microscopi':10 'mitot':15,51 'number':33 'primari':64,77 'prolifer':45 'quantifi':28 'rate':46 'respect':32 'sampl':80 'stem':60,72 'tissu':59,65,71,78 'uncultur':76 'use':36

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