ULTRA DEEP SEQUENCING

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The sequencing of genomic materials or amplicons (i;e;, PCR products) for the identification and quantitation of rare sequences in a complex mixture; The ability to sequence ultra deep derives from the 'instant cloning' technique that occurs in a proprietary 'emulsion amplification 'process' provided by instrumentation manufactured by 454 Corporation, and the very high number of sequencing reads that can be generated in a short time from the 'clones; When sequenced on the 454 Corporation instruments, each amplicon clone is sequenced individually allowing for the identification of rare variants and the assignment of haplotype over the full sequence length